ITS regions, or virulence
genes, have been used to design specific PCR primers and probes to
detect and discriminate between species of bacteria (Tilsala-
Timisj€arvi and Alatossava, 1997), such as Staphylococcus aureus
(Kadiroglu et al., 2014), Enterococcus sp., and Escherichia coli (Noble
et al., 2010). The most reported PCR methods for bacteria are based
on the detection of DNA from both live and dead cells