VCO was prepared according to the method reported by Hamid et al. (2011) with slight modification. The fresh endosperm of mature coconut was grated and made into viscous slurry and then squeezed using a cheese cloth to obtain coconut milk. The coconut milk was chilled to 10 C to break the emulsion and then centrifuged at 830g for 5 min to obtain raw coconut cream, skim milk,and some proteins or solid components. The coconut cream was kept at 4 C for 24 h and then subjected to mild heating (65 C)in a thermostat oven until the cream temperature reached 65 C,followed by centrifugation (2306g for 10 min) to separate non-oilfractions from the oil phase. Finally, the raw oil was filteredthrough Whatman filter paper to obtain VCO. The obtained VCOwas labeled VCO-1 and used for the adulteration study