The instrumental limits of detection (3Sy/x/b criteria) and quantification (10Sy/x/b criteria) values were 0.02 and 0.07 μg mL−1 for hydroxytyrosol, respectively; 0.06 and 0.18 μg mL−1 for tyrosol, respectively. These low limits of detection and quantification along with the broad linear range observed, allow the determination of the target analytes in a variety of different samples, containing different amounts of them.