Abstract Previous work found that lipoxygenases were
key enzymes in lipid peroxidation, which causes grain
deterioration during storage. In order to obtain better seed
viability in rice, 10 marker-free knock-down lines were
obtained in the progeny of endogenous knock-down
mutations caused by the RNAi technology. After artificial
accelerated aging, there were four types of knock-down lines
with higher seed viability than wild type (receptor parent).
3 knock-down line was of special interest. In
a series of experiments, including Southern blots, analysis of
expression, and enzymatic activity, had
better seed viability than wild-type. We also investigated the
main agronomic characters of both knock-down lines and
non-transgenetic wild type families. Knock-down lines were
identified with generally excellent agronomic characteristics
similar to the wild-type.