The International Honey Commission (IHC) collaboratively tested three methods for the determination of HMF: two are spectrophotometrical methods (Winkler’s [17] and White’s methods [18]), whereas the thirdwas a RP-HPLC [19] method. Comparison of their performance was reported by Zappalà et al. [20]. Spectrophotometric methods are fast but low on specificity and sensitivity, on the other hand, the RP-HPLCmethod is more accurate and sensitive than the spectrophotometric ones, but is quite slow. In addition, the application of this method to HMF evaluation in strawberry tree honey samples has recently evidenced a specific and heavy bias [21].