The antioxidant activity was obtained through a modified
ABTS+ free radical capture method (Re et al., 1999). The cation
ABTS+ radical was generated through the reaction of the ABTS
stock solution 7 mM with 140 mM of potassium persulfate leaving
it in the dark at room temperature for 16 h before use. The ABTS+
radical was diluted in ethanol for an absorbance of 0.70 ± 0.05 at
734 nm. The spectrophotometric reading was made after exactly
6 min from the mixture of 30 lL of the extract with 3 ml of the radical.
As a standard solution, it was used the Trolox synthetic antioxidant
with a 100–2000 lM concentration in ethanol. The results
were expressed in lMolar of Trolox/g.