2.8. Limits of detection (LOD) and quantification (LOQ)
The limits of detection and quantification were determined
by serial dilutions of ascorbic acid solutions in order to obtain
signal/noise ratios of ≈3:1 for LOD and ≈10:1 for LOQ.
Approximately 25 mg of standard ascorbic acid were
weighed precisely and dissolved in 50mL of the mobile
phase. Appropriate amounts of standard ascorbic acid solution
were diluted to required concentrations of 0.03, 0.05, 0.1
and 1.0gmL−1.Working standard solutions were prepared in
replicates of three [21,25–27].