Fat was determined according to the method of AOAO (1984)
using soxhlet apparatus as follows: An empty clean and dry exhaustion
flask was weighed, about 2 g of sample was weighed and placed in a lean
extraction thimble and covered with cotton wool. The thimble was placed
in an extractor. Extraction was carried out for 6 – 8 h with petroleum
ether. The heat was regulated to obtain at least 1 siphoning per hour. The
residual ether was dried by evaporation. The extraction flask was placed
in an oven till it dried completely and then cooled in a desiccators and
weighed. The fat content was calculated using the following equation: