2.6. SEM analysis
The morphological changes in Rhizobium leguminosarum bv. viciae
3841 were imaged using scanning electron microscopy, SEM (Wood
et al., 1989). The detailed procedures have been reported previously
(Fan et al., 2014). Briefly, the samples were transferred into a CO2
critical-point drier and the silicon wafers were placed onto SEM stubs,
which was sputter coated with Au/Pd using a Denton Vacuum Bench
Top Turbo III sputter coater. All samples were visualized and imaged
with a Hitachi S-4700 field-emission SEM.