2.2. Growth conditions
The evaluation of biomass was carried out in an orbital shaker at 150 rpm at
different temperatures and times in 250-mL Erlenmeyer flasks, where each one contained 50 mL of growth medium. The flasks were inoculated with a cell suspension at
5%, v/v with 2.5×106 cells/mL. The biomass increase was determined by gravimetry
and the results were expressed in mg in dry base.