Bacillus sp. strain CaSUT007, a plant growth promoting rhizobacterium isolated from cassava, was
investigated for the secretion of compounds that might be involved in plant growth promotion. Extracts
containing phytohormone and extracellular proteins were made from the cell-free fluid of CaSUT007
broth cultures. These extracts, along with a whole culture of CaSUT007 and the raw fluid and cellular
fractions from a CaSUT007 culture, were applied separately to cassava stakes. The stakes were planted
into pots of soil maintained in a greenhouse condition. Under this condition, all of the extracts
including phytohormones and extracellular proteins increased root and shoot lengths and cassava
biomass as compared to negative control. Our results indicate that the culture extracts, when applied to
cassava stakes, increased root and shoot lengths by more than 30%, and increased fresh and dry
weights by more than 25% compared to the distilled water control. Thus, photohormone and
extracellular proteins secreted by CaSUT007 can influence plant growth and development. Analysis of
the photohormone and extracellular proteins extracts revealed indole-3-acetic acid and peptides to be
the primary compounds.
Bacillus sp. strain CaSUT007, a plant growth promoting rhizobacterium isolated from cassava, wasinvestigated for the secretion of compounds that might be involved in plant growth promotion. Extractscontaining phytohormone and extracellular proteins were made from the cell-free fluid of CaSUT007broth cultures. These extracts, along with a whole culture of CaSUT007 and the raw fluid and cellularfractions from a CaSUT007 culture, were applied separately to cassava stakes. The stakes were plantedinto pots of soil maintained in a greenhouse condition. Under this condition, all of the extractsincluding phytohormones and extracellular proteins increased root and shoot lengths and cassavabiomass as compared to negative control. Our results indicate that the culture extracts, when applied tocassava stakes, increased root and shoot lengths by more than 30%, and increased fresh and dryweights by more than 25% compared to the distilled water control. Thus, photohormone andextracellular proteins secreted by CaSUT007 can influence plant growth and development. Analysis ofthe photohormone and extracellular proteins extracts revealed indole-3-acetic acid and peptides to bethe primary compounds.
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