xylene.
Para⁄n tissue blocks were prepared by using
fresh molten para⁄n and metal ‘L’ blocks and stored
in the refrigerator to avoid brittling of the wax. Desired
blocks were trimmed into a proper size using a
sharp blade and ¢xed on the block holder of aWestoxrotary
microtome. Sections were cut at 5 mm thickness
and ribbons were carefully collected for ¢xation
on albuminized glass slides. After ¢xation, tissues
were stained using the standard haematoxylin^eosin
method, and mounted with a coverslip using DPX
mount. Observation was made in a light microscope
(Olympus CX 31) at 10.Water quality parameters
were also analysed using standard methods (APHA,
AWWA & WEF 1998). Di¡erences in the mean initial
and ¢nal weights were tested by one-way analysis of
variance for signi¢cant di¡erence (Zar1984).