Candida isolates were speciated phenotypically by germ tube test (GTT),11 Rice Tween-80 agar performed as described in previous studies,12 in addition to Chrom ID Candida Agar (CAN2) (BioMérieux, France) and API 20C AUX (BioMérieux, France), which were performed according to manufacturers’ instructions. The Candida isolates were then stored in glycerol broth at −70 °C for further processing by PCR-restriction fragment length polymorphism (PCR-RFLP).