Near infrared spectroscopy, a rapid and accurate biomass analysis technique
Near infrared spectroscopy (NIRS), in which CIRAD has acknowledged expertise, is an analysis technique centring on the principle of near infrared radiation energy absorption. It is used to predict the chemical composition of a sample, but must be precisely calibrated beforehand. This preliminary work requires a series of studies to select and optimize the extraction process, calibrate the analysis for each component and establish the corresponding calibration equations.
For rubber and resin reference determination analyses, the method chosen was accelerated solvent extraction (ASE). The resins were extracted with acetone at 40°C, followed by the polyisoprene using hexane at 120°C.
More than 200 stem and branch samples from several guayule lines harvested in the Montpellier region (France) and Murcia province (Spain) were analysed for both their moisture content and their rubber and resin content. Two thirds of the samples were used to establish the calibration curve and the other to validate the calibration.
As regards moisture content, the values confirmed that the calibration curve was sufficiently accurate for this type of measurement. The values obtained for the extracts, hexane (rubber) and acetone (resins), showed that NIRS enabled accurate characterization of guayule biomass.
Near infrared spectroscopy, a rapid and accurate biomass analysis techniqueNear infrared spectroscopy (NIRS), in which CIRAD has acknowledged expertise, is an analysis technique centring on the principle of near infrared radiation energy absorption. It is used to predict the chemical composition of a sample, but must be precisely calibrated beforehand. This preliminary work requires a series of studies to select and optimize the extraction process, calibrate the analysis for each component and establish the corresponding calibration equations.For rubber and resin reference determination analyses, the method chosen was accelerated solvent extraction (ASE). The resins were extracted with acetone at 40°C, followed by the polyisoprene using hexane at 120°C.More than 200 stem and branch samples from several guayule lines harvested in the Montpellier region (France) and Murcia province (Spain) were analysed for both their moisture content and their rubber and resin content. Two thirds of the samples were used to establish the calibration curve and the other to validate the calibration.As regards moisture content, the values confirmed that the calibration curve was sufficiently accurate for this type of measurement. The values obtained for the extracts, hexane (rubber) and acetone (resins), showed that NIRS enabled accurate characterization of guayule biomass.
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