In order to identify the principle compound present in the
Zimmu leaf extract, the extract was subject to solvent extraction,
purification by TLC followed by GCeMS analysis. From these analyses,
two lipid compounds exhibiting significant effect on the
mycelial growth and spore germination of the pathogen
M. eumusae were purified and the GCeMS analysis of these
compounds indicated the presence of up to six different
components and the further research work on these components
is in progress for the wide use of this plant extract. Muthukumar
et al. (2010) also reported the presence of 22 compounds in
Zimmu leaf extract and opined that among these, the lipid compound
n-Hexadecanoic acid may be responsible for the inhibition
of the growth of test pathogen Pythium aphanidermatum. Conversely,
Satya et al. (2005) reported that the phenolic compounds