Therefore, 10 mL samples of water from each treatment were collected
every five days of the experiment, using 15 mL sterile test tubes with
screw caps for determining the microbial count in the water (counting
viable heterotrophic bacteria and Vibrionaceae). However, 1 mL samples
were inoculated in serial dilutions through saline tubes (3% NaCl)
and 100 μL of the samples was seeded on Petri plates containing bacteriological
culture medium and Marine Agar and Thiosulphate Citrate
Bile Sucrose (TCBS) Agar for counting viable heterotrophic bacteria
and Vibrionaceae, respectively. Later, theywere incubated in a bacterial
incubator at 30 °C for 24 h and counted the next day using themethod of
counting plates on colony forming units (CFU·mL−1).