A comprehensive survey of the literature on the FFAs purification
in food products allowed us to identify a reference method,
developed by Kaluzny, Duncan, Merritt, and Epps (1985), utilising
aminopropyl bonded phase for lipid mixture separation into individual
classes in high yield and purity. This method, together with
the FA methylation, allowed us to obtain a accurate procedure for
the measurement of FFAs in beer wort.