were manually pollinated as described above, then immediately inoculated by depositing 10 μl of a suspension containing AAC00-1ΔhrcC, AAA99-2 at approximately 1 × 108 CFU/ml, or PBS as a negative control onto the stigmas.
were manually pollinated as described above,then immediately inoculated by depositing 10 μl of a suspensioncontaining AAC00-1ΔhrcC, AAA99-2 at approximately 1 × 108CFU/ml, or PBS as a negative control onto the stigmas.