3.2.2. Determination of Median Lethal Dose (LD50)
Oral administration of (70%) alcoholic extract of P. dichotomus in doses up to 4000 mg/Kg b.
wt. failed to kill mice within 24 h. The tested extract is considered highly safe, since substances
possessing LD50 higher than 50 mg/Kg b.wt. are considered non toxic [27].
3.2.3. Anti-inflammatory Activity
It was noticed that (70%) alcoholic extract of P. dichotomus in a dose of 100 mg/Kg b.wt.
significantly decreased the paw thickness in comparison to the control and standard groups. The effect
appeared at 2h and persisted for a period of 3 hours post-administration (Table 5) while a dose of (50
mg/Kg b.wt) has a slightly effect.