As we know, time is one of themost important factors in the formation
of nanoparticles during biosynthesis techniques. As the duration of
reaction increases, more silver nanoparticles are formed. Due to the
instability of the silver nanoparticles formed, an optimum duration is
required, as silver nanoparticle agglomeration after the optimum
duration resulting in larger particle sizes. The optimum time required
for the completion of reaction from our study was 24 h