Nutrient agar media plates were
inoculated with 0.1 ml of 108 CFU/ml broth culture
of tested bacterial suspension and immediately sterile
paper discs impregnated with 30 μl of the cyanobacterial
extracted materials that air dried, were placed over the
agar surface. The plates were incubated at 37 °C for 24
h and examined for zones of inhibition. Discs with the
appropriate solvents used in extraction were used as
control (Bauer et al., 1966).