Results and discussion
The absorption spectrum of carmine shows that maximum
absorbance occurs at 513 nm and the presence of surfactant does
not have significant effect on maximum wavelength. Therefore,
all the absorbance measurements were performed at this wavelength.
Carmine is extracted into non-ionic surfactant, Triton
X-100. The influences of main parameters in CPE method including
pH of the medium, surfactant and salt concentrations, incubation
time and temperature were optimized in order to obtain the highest
sensitivity and recovery.