For trichodinids taxonomic studies, nitric acid is used to remove the soft material and release the adhesive disc The acid is known to soften chitin and keratin, acting as evidence for the presence of these compounds or a similar one on the protozoan’s structure. Thus, the efficacy of TFB may be explained because it is an insecticide which inhibits the synthesis of chitin. However, further research about the characterization of the protozoan structures and the TFB’s mode of action for
trichodinids is required.