. Mass multiplication and preparation of fungal inoculum
The selected phytopathogenic fungi (F. oxysporum, P. aphanidermatum,
P. debaryanum and R. solani) were multiplied using
sorghum seeds [Sorghum bicolor (L.) Moench]. The inoculum of the
fungi was produced on sorghum seeds moistened with water (1:1)
and autoclaved twice for 90 min on two consecutive days (Paulitz and
Schroeder, 2005). Flasks containing sorghum seeds were inoculated
with one week-old fungal culture grown on potato dextrose agar
(PDA) media. Flasks were incubated at room temperature for four
weeks and shacked once aweek. Colonized sorghum seeds were used
as fungal inoculums at a rate of 15 g in 500 g of potting mix.