Bacterial cellulose was extracted from nata-de-coco, by first rinsing the food product
three times with dH2O, the product was then sieved, homogenised and blended using a
variable speed laboratory blender operated at maximum speed (Waring Laboratory,
Essex, UK). The bacterial cellulose was then purified by boiling a concentration of 0.6
w/v % in 0.1M NaOH at 80° C for 2 h to remove any remaining microorganisms and
soluble polysaccharides [10]. Bacterial cellulose was successively centrifuged (15 000 g),
homogenised and rinsed to neutral pH.