Prior to the collection of sperm, 2 sexually mature male specimens of MGC weighing
approximately 25 kg were subjected to two weeks of conditioning and fed with individually
formulated pellet feed fortified with 30% crude protein (at 5% of their body weight per day). The
MGC sperm were first stripped to check for the presence of milt and then the fish were injected with a
gonadotropin-releasing hormone analogue (Suprefact®
, Hoechst AG, Germany) and domperidone
(Olic Limited., Thailand) at a dosage of 10 µg/kg and 5 mg/kg body weight respectively. After 8 hours
after hormone injection, the MGC were stripped of milt. The collected milt was then kept at 15oC