Figure 4: The improvement of filter specificity with PBS wash. (a) Mean red emission RFU values were taken from images of the filter at each point in the wash.
Particle analysis was conducted on images from the green emission channel to determine the number of captured cancer cells at each point in the wash.
(b-d) Representative composite images from (b) before PBS wash, (c) after 8 ml of wash, and (d) after 18 ml of wash.