Liposome suspension with carotenoids was prepared by mixing egg yolk phospholipids (PL) purified by the method of Palacios and Wang (2005). Stock solution of PL in chloroform (final concentration of 2.5 mg/ml) was mixed with 2.5 mg of sample in methanol, evaporated to dryness by flushing with nitrogen. Distilled water (10 ml) was added to the dried film and sonicated for 5 min at 50 Hz, with 1:1 pulse frequency using a sonicator.