Columns one to six and rows A to G of a microtitre
plate (1a) were marked off. A second plate (1b) was
taken, columns were marked off and rows were labeled
as A1/G1. Using an Eppendorf pipette, isosensitised
nutrient broth (90 ml) was added to wells one to six of
rows B to G of plate 1a and to rows A1 to F1 of plate 1b.
To wells 1/4 of row A, the extracts at a concentration of
1 mg/ml were added, and DMSO and ciprofloxacin at a
concentration of 0.25 mg/ml were to rows 5 and 6,
respectively.