Silver nanoparticles (AgNPs) have been used for decades as anti-bacterial agents in various industrial
fields such as cosmetics, health industry, food storage, textile coatings and environmental applications,
although their toxicity is not fully recognized yet. Antimicrobial and catalytic activity of AgNPs depends
on their size as well as structure, shape, size distribution, and physico-chemical environment. The unique
properties of AgNPs require novel or modified toxicological methods for evaluation of their toxic
potential combined with robust analytical methods for characterization of nanoparticles applied in
relevant vehicles, e.g., culture medium with/without serum and phosphate buffered saline.