Then the medium was sterilized by autoclaving at 121 °C and
at 15 lb for 15 min. After sterilization, the medium and sterile
Petri plates were transferred into laminar air flow chamber.
Approximately 15-20 mL of the sterile medium was poured
in each sterile Petri plates. The medium was allowed to
solidify in laminar flow and inoculated with the overnight
bacterial cultures. The sterile Whatman fitter paper No.
1 disc was prepared and discs were soaked in the plant
extracts and then placed over the inoculated plates. After
24 h of incubation at 37 °C, the zone of inhibition around the
disc was measured[7].