A Stabilwax-DA column (30 m 0.32 mm i.d., 0.25 mm film
thickness, Restek, Bellefonte, USA) with a stationary phase bonded
with polyethylene glycol having an acidic functionality incorporated
into the polymer structure was the third column selected
for this study. In the experiment, only 12 fatty acids were
separated and the resolution of stearic acid (C18:0), oleic acid
(C18:1) and linoleic acid (C18:2) was poor. Some of the separated
fatty acids exhibited peak fronting in the chromatograms. Most
likely, the peak fronting can be attributed to the polarity mismatch
between the column stationary phase and the fatty acids.