Cell immobilization
TSCs of mulberry silkworms were purchased from a local silk
factory (Yala, Thailand) (Fig. 1). Cell immobilization was performed
as follows. 2.5 g of TSCs and 250 mL of culture medium in 500 mL
flasks were autoclaved separately for 15 min at 121 C. 10 mL of
stock cell suspension was then added to the sterilized culture medium.
The sterilized TSCs were immersed in the culture medium and
incubated at 150 rpm and 33 C for 24 h to induce natural cell
adhesion.