The culture-dependent analysis was performed immediately
after sampling following the protocol of Camu et al. (2007). Briefly,
180 ml of 0.1% (wt/vol) peptone water (Oxoid, Basingstoke, United
Kingdom) was added to 20 g of pulp and beans in a sterile stomacher
bag that was vigorously shaken for 5 min in a Stomacher 400
(Seward, Worthington, United Kingdom)