3.7. HPLC analysis of lignans from seeds of different varieties of S.indicum L.The HPLC of the ethanol extracts of sesame seeds with narin-genin as internal standard depending on the variety showed thepresence of 7–12 distinct peaks corresponding to as many differentlignans in these samples (Fig. 7). Three of the peaks corresponded tosesamol, sesamin and sesamolin as revealed by their retention timecoinciding with the respective reference standards (Figs. 6 and 7).Concentrations of the three compounds were calculated using theresponse factor and data is presented in Table 2. A significant vari-ability was observed in the content of lignans under considerationin all the three groups.