Bio-protective these cultures inhibited the growth of Brochothrix thermosphacta. Bio-protective cultures, which inhibited the spoilage bacteria, cultures as the odours, flavours, and the sticky white slime, that are indicative of deterioration. were able to reduce the TVB-N to values below 30 mg nitrogen/100 g. Can potentially extend the shelf life up to 12 days and improve the sensory properties of hamburger meat.