FIG. 3. Scanning electron microscope and transesterification. (A) Celite without lipase. (B) Celite with enzyme. (C) Transesterification reaction conditions: Botryococcus sp. oil (1 g)
and methanol with 1:3 molar ratio, water (5 ml), and 0.75 ml of t-butanol; finally, the reactionwas started by adding the immobilized enzyme (8% w/w of oil), and carried out at 37C
and 150 rpm. Data points were means of triplicate values with the error bars showing standard deviations.