For each test, 10 disease-free, laboratory-reared
Aedes aegypti
female mosquitoes that were between 7 and 24 days old were placed
into separate laboratory cages measuring 30 cm by 22 cm by 22 cm.
A batch of 10 mosquitoes that had not been exposed to the repellent
being tested was used for each arm insertion. Mosquitoes were
provided with a constant supply of 5 percent sucrose solution.
Cages were placed in a walk-in incubator measuring 2.2 m by 2.2 m
by 2.2 m, in which the temperature was maintained at 24 to 32°C,
the relative humidity at 60 to 70 percent, and the light–dark cycle
at 12 hours of light followed by 12 hours of darkness.