This chapter describes an Alu PCR method based on the use of two fluorochrome-labelled primers, combined with capillary
electrophoresis analysis of PCR products. The method has been presented in a previous paper (11) and used to detect a new longevity-associated locus (2). This variant of the Alu PCR technique uses two different Alu-specific primers labelled with
different fluorochromes in the same PCR reaction; the resulting PCR products are then separated by capillary electrophoresis and fluorescent detection.