Protein content
2 g ground sample was weighed into a 300 cm3 Kjeldahl digestion flask and few antidumping granules (1.0g of K2S04 and anhydrous CuS04) were added to the flask. 25 cm3 of concentrated sulphuric acid (98% w/w) was added. The flask was fixed on the Kjeldahl digestion jack and heated slowly at first until frothing subsides and then vigorously with occasional rotation, conical flask was titrated with 0.1 mol hydrochloric acid, and an estimate of the crude protein content was calculated by multiplication of the organic nitrogen content by a factor of 6.25. The percentage protein was calculated following the standard methods of the Association of Official Analytical Chemists (1990). The three different samples (AB, GH and OM) were analyzed in triplicate. Total carbohydrate content was calculated from the difference.