Dunaliella tertiolecta is euryhaline unicellular green algae considered as potential candidate for biodiesel production. This is mainly due to its capacity to respond to external stimuli (salt stress, nitrogen depletion, etc.) by increasing its lipid content. Nevertheless, use of D.tertiolecta for biodiesel production is hindered by their slow growth rates. Thereby, for an economic exploitation of D. tertiolecta for biodiesel production two issues have to be optimized: growth rate and lipid content. Unfortunately, only few works reported improvement of lipid content in D. tertiolecta using salt stress while none treated growth stimulation of this specie. In most microalgae, efforts for lipid content improvement by stress application are hampered by the growth inhibition due to limiting conditions in the culture medium. This was also the case of Dunaliella whose growth was inhibited after high NaCl concentrations addition. To overcome this problem; work with two-phase method was developed in some species. This strategy includes a first phase allowing biomass accumulation without stress application and when sufficient biomass is reached stress is applied to enhance lipids accumulation.