The measurements of FSC and BAT collagen mass loss were performed by heating the samples until the mass was fixed, in the temperature range 293 K–383 K. The samples were cooled down and reheated to 473 K. Furthermore, the measurements were performed when heating the samples every 10 K until the temperature reached 473 K (Figs. 9, 10). Collagen mass loss in time, as well as, a relative collagen mass change are shown in Fig. 11 for FSC and in Fig. 12 for BAT collagen. The samples were heated for 15 to 20 minutes at every consecutive temperature, until they reached fixed mass. The aim of the measurements performed up to the temperature of 383 K was to determine the amount of free water. Heating above 383 K, to 473 K, on the other hand, allowed to determine the amount of bound and structural water. To determine the total amount of water in a sample, it was necessary to calculate sample mass at 473 K, since structural water is released when the process of thermal denaturation is initialized. Sample heating to 473 K was restricted by technical capabilities of the HB43-S moisture analyzer. The differences in moisture at 383 K and 473 K were greater for FSC than for BAT collagen and were 9% and 5%, respectively.