The optimal temperature of Phytophthora growth is dependent on the species mentioned above. Each experiment was repeated twice, with three biological replications per experiment. The percent inhibition of mycelial growth was calculated as follows: growth inhibition (%) = [(control mycelia diameter–treated mycelia diameter)/control mycelia diameter] × 100 (Satish et al., 2007). Trichoderma isolates selected through preliminary screening that showed 100% inhibitory activity against at least three Phytophthora species via EtOAc extract with a concentration of 100 μg/10 μL were further tested for anti-Phytophthora activity using: (i) minimum inhibitory concentration (MIC) test using the EtOAc extract, (ii) disk diffusion test using the EtOAc extract, and (iii) antibiosis test using the liquid culture filtrate of potato dextrose broth (PDB).