Molecular masses determination and construction of LDA models
The molecular mass standard mixture solution was injected under the selected CGE conditions, and employed to determine the molecular masses of sample protein peaks. For this purpose, a calibration curve was obtained by plotting the logarithm of the standard protein molecular masses versus the tref/ti ratio, where tref is the migration time of electroosmotic flow (EOF) peak (or reference peak) and ti is the migration time of sample proteins. The obtained calibration curve was described by the following regression equation: log MW = −2.980 (tref/ti) + 2.609 (r = 0.9904).