2.6. Assessment of creaming
Emulsion samples of 20 mL were placed in 20 mL graduated glass vessels. The assessment of creaming was recorded (in mL) through the visible boundary (between continuous phase or the creaming droplets and the upper cream layer) which moved up in the graduated measuring tube during ageing. The boundary evolution was monitored during a 4 h ageing period and until completion of creaming (Moates et al., 2001 and Chanamai and McClements, 2000a). A blue dye ( E131 at 0.001% (w/w) ) was added to the emulsions to facilitate the observation of the boundary. It was first verified that this dye had no effect on emulsion properties (results not shown).