The effect of RELM in the reduction of a solid tumor induced by DLA cells is shown in Fig. 2.
2.5. Effect of RELM extract on solid and ascites tumor development
Dalton's lymphoma ascite (DLA) cell lines (106 cells/animal) were injected subcutaneously into the right hind limb of Swiss albino mice for solid tumor development and divided into five groups containing six mice in each group [21]. Group I served as tumor control and received CMC (0.1%). Group II was treated with cyclophosphamide (10 mg/kg b. wt). Groups III, IV, and V were treated with RELM extract at 50 mg/kg b. wt, 100 mg/kg b. wt, and 250 mg/kg b. wt doses, respectively. The treatments were administered orally after 24 hours of DLA cell inoculation and continued once daily for 10 days. The initial diameter of the right hind limb was noted using vernier calipers. The tumor volume (V) was measured every 3rd day and recorded up to 1 month: