Materials and reagents
The test drug was eperisone hydrochloride tablet containing
50 mg of eperisone hydrochloride per tablet, which was
obtained from Eisai (China) Pharmaceutical Co. Ltd. (Suzhou,
China). Methanol was of HPLC grade (Merck KGaA). Acetic
acid, ammonium acetate, diethyl ether, hydrochloric acid and
sodium bicarbonate were analytical grade purity and purchased
from Nanjing Chemical Reagent Co. Ltd. (Nanjing, China)