To extract DNA, a ~1 mm yeast colony from the PDA plate was
added to 50 mL of sterile deionised water, boiled at 95 C for 10 min
and centrifuged at 16000 g for 5 min. ITS region amplifications were
performed using the primers ITS1 (50-TCCGTAGGTGAACCTGCGG-
30) and ITS4 (50-TCCTCCGCTTATTGATATGC-30),