2.2 Fermentation
About 30 mL of activated Monascus sp. broth (2 x 24 hours) was inoculated aseptically to 300 g of sterile rice in
a bottle under Laminar Air Flow (LAF). The inoculated rice were shaken until homogen and incubated for 14 days
in incubator of 30 o
C. Monascus Fermented Rice (MFR) was moisturized using sugar solution (@4 mL) every 24
hours for the first of 7 days. The shakes of MFR were performed once a day until incubation time completed. On the
14th day MFR were harvested and dried in oven of 50 o
C for 24 hours then blended before extraction.