Metal Chelating Assay
Measuring the ability to bind to the metal Residents of the measure the absorbance at that wavelength.
562 nm with the decreased By Adding Ferrozine this substance going to catch Fe2+ and in the format Ferrozine - Fe2+complex which will be red ,
if want to have the ability to test the Fe2 + binds to the The Antioxidant - Fe2 + complex and will cause red Ferrozine - Fe2 + complex lighter
EDTA
Ethylene diamine tetraacetic acid is qualified Chelating agent by binding with metal ions such as lead, iron, zinc, cadmium ,manganese and Copper can be used disposal of metal ions